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Agarose and acrylamide powders, buffers, diluents, markers and stains for protein/nucleic acid electrophoresis; featuring concentration, grade, melting point, capacity to stabilize, control concentration, denature, reduce, and mark target molecules.
A selective inhibitor of the reverse mode of NCX1 (IC50 = 1.2-2.4 µM) that prevents intracellular sodium-dependent calcium uptake in whole cells; exhibits neuroprotection from glutamate-induced excitotoxicity; inhibits TRPC channels involved in calcium-dependent signal transduction
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SYPRO Ruby protein gel stain is a ready-to-use ultrasensitive luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain designed especially for use in 2-D PAGE has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEf) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However unlike silver staining the SYPRO Ruby gel stainuses a simple staining protocol with no possibility of overstainingdelivers a linear quantitation range of over three orders of magnitudeshows less protein-to-protein variabilitystains glycoproteins lipoproteins calcium-binding proteins fibrillar proteins and other difficult-to-stain proteinswill not stain extraneous nucleic acidsdoes not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
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Sigmas DirectLoad(TM) 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb. for NA electrophoresis 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.
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Sigmas PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 - 1 000 bases evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
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